|
荧光染料
|
吸收波长
|
发射波长
|
Suggested
Laser
|
|
Acid Fuchsin
|
|
|
Green He-Ne
|
|
ACMA (Aminochloromethoxyacridine)
|
|
|
Blue Diode
|
Acridine Orange
(Bound to DNA)
|
|
|
Argon-Ion
|
|
Acridine Red
|
|
|
Argon-Ion
|
|
Acridine Yellow
|
|
|
Argon-Ion
|
|
Acriflavin
|
|
|
Blue Diode
|
|
AFA (Acriflavin Feulgen SITSA)
|
|
|
Blue Diode
|
|
Alexa Fluor 350
|
|
|
Helium-Cadmium
|
|
Alexa Fluor 405
|
|
|
Blue Diode
|
|
Alexa Fluor 430
|
|
|
Blue Diode
|
|
Alexa Fluor 488
|
|
|
Argon-Ion
|
|
Alexa Fluor 500
|
|
|
Argon-Ion
|
|
Alexa Fluor 514
|
|
|
Argon-Ion
|
|
Alexa Fluor 532
|
|
|
Krypton-Argon
|
|
Alexa Fluor 546
|
|
|
Green He-Ne
|
|
Alexa Fluor 555
|
|
|
Krypton-Argon
|
|
Alexa Fluor 568
|
|
|
Krypton-Argon
|
|
Alexa Fluor 594
|
|
|
Orange He-Ne
|
|
Alexa Fluor 610
|
|
|
Orange He-Ne
|
|
Alexa Fluor 633
|
|
|
Red He-Ne
|
|
Alexa Fluor 647
|
|
|
Krypton-Argon
|
|
Alexa Fluor 660
|
|
|
Red Diode
|
|
Alexa Fluor 680
|
|
|
Red Diode
|
|
Alizarin Complexon
|
|
|
Green He-Ne
|
|
Alizarin Red
|
|
|
Green He-Ne
|
|
AMCA
|
|
|
Helium-Cadmium
|
|
Aminoactinomycin D
|
|
|
Krypton-Argon
|
|
6-Aminoquinoline
|
|
|
Helium-Cadmium
|
|
Aniline Blue
|
|
|
Argon-Ion
|
|
ANS (Anilinonaphthalene Sulfonic Acid)
|
|
|
Blue Diode
|
|
Anthroyl Stearate
|
|
|
Argon-Ion
|
|
APC (Allophycocyanin)
|
|
|
Krypton-Argon
|
|
Astrazon Brilliant Red 4G
|
|
|
Argon-Ion
|
|
Astrazon Orange R
|
|
|
Argon-Ion
|
|
Astrazon Red 6B
|
|
|
Argon-Ion
|
|
Astrazon Yellow 7 GLL
|
|
|
Argon-Ion
|
|
Atabrine
|
|
|
Blue Diode
|
|
ATTO-TAG CBQ
|
|
|
Argon-Ion
|
|
ATTO-TAG FQ
|
|
|
Argon-Ion
|
|
Auramine O
|
|
|
Argon-Ion
|
|
Aurophosphine
|
|
|
Argon-Ion
|
|
Aurophosphine G
|
|
|
Argon-Ion
|
|
BAO 9-(Bisaminophenyloxadiazole)
|
|
|
Argon-Ion
|
|
BCECF
|
|
|
Argon-Ion
|
|
Berberine Sulphate
|
|
|
Blue Diode
|
|
Bisbenzamide
|
|
|
Helium-Cadmium
|
|
BOBO-1, BO-PRO-1
|
|
|
Argon-Ion
|
|
BOBO-3, BO-PRO-3
|
|
|
Krypton-Argon
|
|
Blancophor FFG Solution
|
|
|
Blue Diode
|
|
Blancophor SV
|
|
|
Argon-Ion
|
|
BODIPY FL
|
|
|
Argon-Ion
|
|
BODIPY TMR
|
|
|
Green He-Ne
|
|
BODIPY TR
|
|
|
Yellow He-Ne
|
|
BODIPY 492/515
|
|
|
Argon-Ion
|
|
BODIPY 493/503
|
|
|
Argon-Ion
|
|
BODIPY 500/510
|
|
|
Argon-Ion
|
|
BODIPY 505/515
|
|
|
Argon-Ion
|
|
BODIPY 530/550
|
|
|
Krypton-Argon
|
|
BODIPY 558/568
|
|
|
Krypton-Argon
|
|
BODIPY 564/570
|
|
|
Krypton-Argon
|
|
BODIPY 581/591
|
|
|
Yellow He-Ne
|
|
BODIPY 630/650
|
|
|
Red He-Ne
|
|
BODIPY 650/665
|
|
|
Red He-Ne
|
|
BODIPY 665/676
|
|
|
Red Diode
|
|
Brilliant Sulphoflavin FF
|
|
|
Blue Diode
|
|
BTC (Benzothiazole Calcium Indicator)
|
|
|
Blue Diode
|
|
Calcein
|
|
|
Argon-Ion
|
|
Calcein Blue
|
|
|
Argon-Ion
|
|
Calcium Crimson
|
|
|
Yellow He-Ne
|
|
Calcium Green
|
|
|
Argon-Ion
|
|
Calcium Orange
|
|
|
Green He-Ne
|
|
Calcofluor (RW Solution)
|
|
|
Argon-Ion
|
|
Calcofluor White
|
|
|
Blue Diode
|
|
Calcofluor White (ABT Solution)
|
|
|
Argon-Ion
|
|
Calcofluor White (Standard Solution)
|
|
|
Argon-Ion
|
|
Carbocyanine LDL
|
|
|
Argon-Ion
|
|
5-(and 6-)-Carboxy SNARF-1 Indicator
|
|
548 (Low pH)
|
|
|
576 (High pH)
|
|
|
|
587 (Low pH)
|
|
|
635 (High pH)
|
|
|
Krypton-Argon
|
|
5-Carboxynaphthofluorescein
|
|
|
Yellow He-Ne
|
|
6-Carboxyrhodamine 6G
|
|
|
Argon-Ion
|
|
Cascade Blue
|
|
|
Blue Diode
|
|
Cascade Yellow
|
|
|
Blue Diode
|
|
Catecholamine
|
|
|
Blue Diode
|
|
CCF2 (GeneBLAzer)
|
|
|
Blue Diode
|
|
CellTracker Blue
|
|
|
Helium-Cadmium
|
|
CellTracker Green BODIPY
|
|
|
Argon-Ion
|
|
CFDA (Carboxyfluorescein Diacetate)
|
|
|
Argon-Ion
|
|
Chinacrine
|
|
|
Argon-Ion
|
|
Chromomycin A3
|
|
|
Argon-Ion
|
|
CL-NERF
|
|
504 (Low pH)
|
|
|
514 (High pH)
|
|
|
|
587 (Low pH)
|
|
|
540 (High pH)
|
|
|
Argon-Ion
|
|
Coriphosphine O
|
|
|
Argon-Ion
|
|
Coumarin-Phalloidin
|
|
|
Blue Diode
|
|
CPM (Coumarin-Maleimide)
|
|
|
Blue Diode
|
|
CTC Formazan
|
|
|
Argon-Ion
|
|
Cy2
|
|
|
Argon-Ion
|
|
Cy3
|
|
|
Green He-Ne
|
|
Cy3.1.8
|
|
|
Krypton-Argon
|
|
Cy3.5
|
|
|
Krypton-Argon
|
|
Cy5
|
|
|
Krypton-Argon
|
|
Cy5.1.8
|
|
|
Krypton-Argon
|
|
Cy5.5
|
|
|
Red Diode
|
|
Cy7
|
|
|
Near-Infrared
|
|
DANS
|
|
|
Helium-Cadmium
|
|
DANSA
|
|
|
Helium-Cadmium
|
|
Dansyl Cadaverine
|
|
|
Helium-Cadmium
|
|
Dansyl Chloride
|
|
|
Helium-Cadmium
|
|
Dansyl Hydrazine
|
|
|
Helium-Cadmium
|
DAO
(Diaminophenyloxydiazole)
|
|
|
Helium-Cadmium
|
|
DAPI
|
|
|
Helium-Cadmium
|
|
Dapoxyl
|
|
|
Argon-Ion
|
|
Di-4-ANEPPS
|
|
|
Argon-Ion
|
|
Di-8-ANEPPS
|
|
|
Argon-Ion
|
|
DiA
|
|
|
Argon-Ion
|
|
DiD (DilC18(5))
|
|
|
Krypton-Argon
|
|
DIDS
|
|
|
Helium-Cadmium
|
|
DiI-C18(3)
|
|
|
Green He-Ne
|
Dimethylamino-5-
Sulphonic Acid
|
|
|
Helium-Cadmium
|
|
DiO-C18(3)
|
|
|
Argon-Ion
|
|
Diphenyl Brilliant Flavine 7GFF
|
|
|
Blue Diode
|
|
DM-NERF
|
|
497 (Low pH)
|
|
|
510 (High pH)
|
|
|
|
527 (Low pH)
|
|
|
536 (High pH)
|
|
|
Argon-Ion
|
|
DPH (Diphenylhexatriene)
|
|
|
Helium-Cadmium
|
|
Dopamine
|
|
|
Helium-Cadmium
|
|
DsRFP (Ds Red Fluorescent Protein)
|
|
|
Green He-Ne
|
|
ELF-97 Alcohol
|
|
|
Helium-Cadmium
|
|
EBFP (Enhanced Blue FP)
|
|
|
Blue Diode
|
|
ECFP (Enhanced Cyan FP)
|
|
|
Blue Diode
|
|
EGFP (Enhanced GFP)
|
|
|
Argon-Ion
|
|
ERTracker Blue/White DPX
|
|
|
Argon-Ion
|
|
EYFP (Enhanced Yellow FP)
|
|
|
Argon-Ion
|
|
Eosin
|
|
|
Argon-Ion
|
|
Erythrosin Isothiocyanate
|
|
|
Argon-Ion
|
|
Ethidium Bromide
|
|
|
Argon-Ion
|
|
Ethidium Homodimer
|
|
|
Argon-Ion
|
|
Euchrysin
|
|
|
Blue Diode
|
|
Evans Blue
|
|
|
Green He-Ne
|
|
FAM (5-Carboxyfluorescein)
|
|
|
Argon-Ion
|
|
Fast Blue
|
|
|
Helium-Cadmium
|
|
FDA (Fluorescein Diacetate)
|
|
|
Blue Diode
|
|
Flazo Orange
|
|
|
Argon-Ion
|
|
Fluorescamine
|
|
|
Blue Diode
|
|
Fluorescein
|
|
|
Argon-Ion
|
|
Fluorescein Isothiocyanate (FITC)
|
|
|
Argon-Ion
|
|
Fluo-3
|
|
|
Argon-Ion
|
|
Fluo-4
|
|
|
Argon-Ion
|
|
FluoroEmerald
|
|
|
Argon-Ion
|
|
FluoroGold (Hydroxystilbamidine)
|
|
323 (Low pH)
|
|
|
368 (High pH)
|
|
|
|
408 (Low pH)
|
|
|
565 (High pH)
|
|
|
Helium-Cadmium
|
|
FluoroJade
|
|
|
Blue Diode
|
|
FluoroRuby
|
|
|
Krypton-Argon
|
|
FluorX
|
|
|
Argon-Ion
|
|
FM1-43
|
|
|
Argon-Ion
|
|
FM4-64
|
|
|
Argon-Ion
|
|
Formaldehyde Induced Fluorescence
|
|
|
Blue Diode
|
|
Fura-2
|
|
363 (Low [Ca])
|
|
|
335 (High [Ca])
|
|
|
|
512 (Low [Ca])
|
|
|
505 (High [Ca])
|
|
|
Helium-Cadmium
|
|
Fura Red
|
|
472 (Low [Ca])
|
|
|
436 (High [Ca])
|
|
|
|
657 (Low [Ca])
|
|
|
637 (High [Ca])
|
|
|
Argon-Ion
|
|
Genacryl Brilliant Red B
|
|
|
Argon-Ion
|
|
Genacryl Brilliant Yellow 10GF
|
|
|
Blue Diode
|
|
Genacryl Pink 3G
|
|
|
Argon-Ion
|
|
Genacryl Yellow 5GF
|
|
|
Blue Diode
|
GFP
(Green Fluorescent Protein)
|
|
|
Blue Diode
|
|
Gloxalic Acid
|
|
|
Blue Diode
|
|
Granular Blue
|
|
|
Helium-Cadmium
|
|
Haematoporphyrin
|
|
|
Green He-Ne
|
Hoechst 33258, 33342
(Bound to DNA)
|
|
|
Helium-Cadmium
|
|
3-Hydroxypyrene-5,8,10-TriSulfonic Acid
|
|
|
Blue Diode
|
|
7-Hydroxy-4-methylcoumarin
|
|
|
Helium-Cadmium
|
|
5-Hydroxy-Tryptamine (5-HT)
|
|
|
Blue Diode
|
|
1,5-IAEDANS
|
|
|
Helium-Cadmium
|
|
Indo-1
|
|
|
Helium-Cadmium
|
|
Indodicarbocyanine
|
|
|
Krypton-Argon
|
|
Intrawhite Cf (Liquid)
|
|
|
Helium-Cadmium
|
|
JC-1
|
|
|
Argon-Ion
|
|
6-JOE
|
|
|
Argon-Ion
|
|
JOJO-1, JO-PRO-1
|
|
|
Argon-Ion
|
|
Leucophor PAF
|
|
|
Argon-Ion
|
|
Leucophor SF
|
|
|
Argon-Ion
|
|
Leucophor WS
|
|
|
Blue Diode
|
|
Lissamine Rhodamine B200 (RD200)
|
|
|
Krypton-Argon
|
|
LOLO-1, LO-PRO-1
|
|
|
Krypton-Argon
|
|
Lucifer Yellow CH
|
|
|
Blue Diode
|
|
Lucifer Yellow VS
|
|
|
Blue Diode
|
|
LysoSensor Blue DND-192, DND-167
|
|
|
Argon-Ion
|
|
LysoSensor Green DND-153, DND-189
|
|
|
Argon-Ion
|
|
LysoSensor Yellow/Blue
|
|
384 (Low pH)
|
|
|
329 (High pH)
|
|
|
|
540 (Low pH)
|
|
|
440 (High pH)
|
|
|
Helium-Cadmium
|
|
LysoTracker Blue
|
|
|
Argon-Ion
|
|
LysoTracker Blue/White
|
|
|
Argon-Ion
|
|
LysoTracker Green
|
|
|
Argon-Ion
|
|
LysoTracker Red
|
|
|
Krypton-Argon
|
|
LysoTracker Yellow HCK-123
|
|
|
Green He-Ne
|
|
Magdala Red
|
|
|
Argon-Ion
|
|
Magnesium Green
|
|
|
Argon-Ion
|
|
Magnesium Orange
|
|
|
Green He-Ne
|
|
Marina Blue
|
|
|
Argon-Ion
|
|
Maxilon Brilliant Flavin 10 GFF
|
|
|
Argon-Ion
|
|
Maxilon Brilliant Flavin 8 GFF
|
|
|
Argon-Ion
|
|
Merocyanine 540
|
|
|
Argon-Ion
|
|
4-Methylumbelliferone
|
|
|
Helium-Cadmium
|
|
Mithramycin
|
|
|
Argon-Ion
|
|
MitoFluor Green
|
|
|
Argon-Ion
|
|
MitoFluor Red 589
|
|
|
Yellow He-Ne
|
|
MitoFluor Red 594
|
|
|
Orange He-Ne
|
|
MitoFluor Far Red 680
|
|
|
Red Diode
|
|
MitoTracker Green FM
|
|
|
Argon-Ion
|
|
MitoTracker Orange CMTMRos
|
|
|
Krypton-Argon
|
|
MitoTracker Red CMXRos
|
|
|
Krypton-Argon
|
|
MitoTracker Red 580
|
|
|
Krypton-Argon
|
|
MitoTracker Deep Red 633
|
|
|
Krypton-Argon
|
|
MPS (Methyl Green Pyronine Stilbene)
|
|
|
Argon-Ion
|
|
Monochlorobimane
|
|
|
Blue Diode
|
|
Nano Orange
|
|
|
Argon-Ion
|
|
NBD
|
|
|
Argon-Ion
|
|
Nile Red
|
|
|
Argon-Ion
|
|
Nitrobenzoxadidole
|
|
|
Argon-Ion
|
|
Noradrenaline
|
|
|
Helium-Cadmium
|
|
Nuclear Fast Red
|
|
|
Argon-Ion
|
|
Nuclear Yellow
|
|
|
Argon-Ion
|
|
Nylosan Brilliant Flavin E8G
|
|
|
Argon-Ion
|
|
Olivomycin
|
|
|
Blue Diode
|
|
OPT (o-Phthalaldehyde)
|
|
|
Helium-Cadmium
|
|
Oregon Green 488
|
|
|
Argon-Ion
|
|
Oregon Green 500
|
|
|
Argon-Ion
|
|
Oregon Green 514
|
|
|
Argon-Ion
|
|
Pacific Blue
|
|
|
Blue Diode
|
|
Pararosaniline (Feulgen)
|
|
|
Krypton-Argon
|
|
PE-B (Phycoerythrin B)
|
|
|
Green He-Ne
|
|
PE-R (Phycoerythrin R)
|
|
|
Argon-Ion
|
|
Phorwite AR (Solution)
|
|
|
Helium-Cadmium
|
|
Phorwite BKL
|
|
|
Argon-Ion
|
|
Phorwite Rev
|
|
|
Argon-Ion
|
|
Phorwite RPA
|
|
|
Argon-Ion
|
|
Phosphine 3R
|
|
|
Argon-Ion
|
|
Phosphine R
|
|
|
Argon-Ion
|
|
Phycocyanin C
|
|
|
Orange He-Ne
|
|
Phycocyanin R
|
|
|
Orange He-Ne
|
|
PKH26
|
|
|
Green He-Ne
|
|
Pontochrome Blue Black
|
|
|
Green He-Ne
|
|
POPO-1, PO-PRO-1
|
|
|
Blue Diode
|
|
POPO-3, PO-PRO-3
|
|
|
Green He-Ne
|
|
Primuline O
|
|
|
Blue Diode
|
|
Procion Yellow
|
|
|
Argon-Ion
|
|
Propidium Iodide
|
|
|
Green He-Ne
|
|
PyMPO
|
|
|
Blue Diode
|
|
Pyrene
|
|
|
Helium-Cadmium
|
|
Pyronin Y
|
|
|
Green He-Ne
|
|
Pyronine
|
|
|
Blue Diode
|
|
Pyronine B
|
|
|
Krypton-Argon
|
|
Pyrozal Brilliant Flavin 7GF
|
|
|
Argon-Ion
|
|
Qdot 525
|
|
|
Blue Diode
|
|
Qdot 525
|
|
|
Blue Diode
|
|
Qdot 585
|
|
|
Blue Diode
|
|
Qdot 605
|
|
|
Blue Diode
|
|
Qdot 655
|
|
|
Blue Diode
|
|
Quinacrine Mustard
|
|
|
Blue Diode
|
|
Resorufin
|
|
|
Krypton-Argon
|
|
RH 414
|
|
|
Argon-Ion
|
|
Rhod-2
|
|
|
Green He-Ne
|
|
Rhodamine 110
|
|
|
Argon-Ion
|
|
Rhodamine 123
|
|
|
Argon-Ion
|
|
Rhodamine 5 GLD
|
|
|
Argon-Ion
|
|
Rhodamine 6G
|
|
|
Argon-Ion
|
|
Rhodamine B
|
|
|
Green He-Ne
|
|
Rhodamine B 200
|
|
|
Green He-Ne
|
|
Rhodamine B Extra
|
|
|
Green He-Ne
|
|
Rhodamine BB
|
|
|
Green He-Ne
|
|
Rhodamine BG
|
|
|
Green He-Ne
|
|
Rhodamine Green
|
|
|
Argon-Ion
|
|
Rhodamine Red
|
|
|
Krypton-Argon
|
|
Rhodamine WT
|
|
|
Argon-Ion
|
|
Rhodol Green
|
|
|
Argon-Ion
|
|
RITC (Rhodamine B Isothiocyanate)
|
|
|
Krypton-Argon
|
|
Rose Bengal
|
|
|
Green He-Ne
|
|
ROX (Carboxy-X-Rhodamine)
|
|
|
Krypton-Argon
|
|
SBFI
|
|
|
Helium-Cadmium
|
|
Serotonin
|
|
|
Argon-Ion
|
|
Sevron Brilliant Red 2B
|
|
|
Argon-Ion
|
|
Sevron Brilliant Red 4G
|
|
|
Argon-Ion
|
|
Sevron Brilliant Red B
|
|
|
Argon-Ion
|
|
Sevron Orange
|
|
|
Blue Diode
|
|
Sevron Yellow L
|
|
|
Blue Diode
|
|
SITS (Stilbeneisothiosulphonic Acid)
|
|
|
Helium-Cadmium
|
|
Sodium Green
|
|
|
Argon-Ion
|
|
Spectrum Aqua
|
|
|
Blue Diode
|
|
Spectrum Green
|
|
|
Argon-Ion
|
|
Spectrum Orange
|
|
|
Krypton-Argon
|
|
Spectrum Red
|
|
|
Orange He-Ne
|
|
Stilbene
|
|
|
Helium-Cadmium
|
|
Sulforhodamine 101
|
|
|
Yellow He-Ne
|
|
Sulforhodamine B
|
|
|
Krypton-Argon
|
|
Sulforhodamine G
|
|
|
Argon-Ion
|
|
SYTOX Blue
|
|
|
Blue Diode
|
|
SYTOX Green
|
|
|
Argon-Ion
|
|
SYTOX Orange
|
|
|
Green He-Ne
|
|
TAMRA (Carboxytetramethylrhodamine)
|
|
|
Green He-Ne
|
|
Tetracycline
|
|
|
Blue Diode
|
|
Texas Red
|
|
|
Yellow He-Ne
|
|
Thiazine Red R
|
|
|
Argon-Ion
|
|
Thiazole Orange
|
|
|
Argon-Ion
|
|
Thioflavin S
|
|
|
Blue Diode
|
|
Thioflavin TCN
|
|
|
Helium-Cadmium
|
|
Thiolyte
|
|
|
Argon-Ion
|
|
Tinopol CBS
|
|
|
Blue Diode
|
|
TOTO 1, TO-RRO-1
|
|
|
Argon-Ion
|
|
TOTO 3, TO-PRO-3
|
|
|
Krypton-Argon
|
|
TRITC
|
|
|
Krypton-Argon
|
|
True Blue
|
|
|
Argon-Ion
|
|
Ultralite
|
|
|
Krypton-Argon
|
|
Uranine B
|
|
|
Blue Diode
|
|
Uvitex SFC
|
|
|
Argon-Ion
|
|
X-Rhodamine
|
|
|
Krypton-Argon
|
|
Xylenol Orange
|
|
|
Green He-Ne
|
|
XRITC (X-Rhodamine Isothiocyanate)
|
|
|
Krypton-Argon
|
|
YOYO-1, YO-PRO-1
|
|
|
Argon-Ion
|
|
YOYO-3, YO-PRO-3
|
|
|
Orange He-Ne
|
